Fig. 7

Target genes prediction and pathway enrichment analysis of the multi-miRNA panel A: Upset plot of 4-miRNA’s target genes via Targetscan, miRWalk, and Tothill dataset. B: Expression heatmap of representative 4-miRNA related target genes between R0 (n = 38) and non-R0 (n = 127) groups in the Tothill dataset. Orange represented up-regulated target genes, and yellow represented down-regulated target genes. C: GO enrichment analysis of target genes was performed and visualized by GOplot. Log(FC) of selected genes was taken from Tothill dataset. Z-score indicated if the biological process (biological process/cellular components/molecular function) was more likely to be increased (Z-score > 0) or decreased (Z-score < 0). The area of the circles was proportional to the number of genes in the pathway. A threshold was set as log(adj P value) > 2. D: A bubble plot of enriched KEGG pathway. E: GSEA was performed by the expression of 4-miRNA target genes between R0 and non-R0 groups in Tothill dataset. miRNA microRNA, R0 advanced ovarian cancer with no residual disease; non-R0 advanced ovarian cancer with any residual disease; KEGG Kyoto Encyclopedia of genes and Genomes