Fig. 2

Characterization of large and small EVs isolated from AOC tissues. A: 10 micro-liters of large a and small vesicles b from AOC tissues were loaded onto grids, negative stained, and evaluated with transmission electron microscopy (TEM). Scale bars, 200 nm. B: Size distribution of large a and small vesicles b were obtained using nanoparticle tracking analysis (NTA; ZetaView^®). Size distribution is presented as graphs with the concentration of the structures on the y-axis and the diameter of the structures in nanometres on the x-axis. C: RNA of large a and small vesicles b were isolated directly from the EV pellets and was analysed with a Bioanalyzer^® (Agilent 2100). AOC advanced ovarian cancer