Fig. 3

a Effect of omentin-1 on hepatocyte fat accumulation. b, c Effect of omentin-1 and FFA co-treatment on the mRNA expression and supernatant release of TNF-α in Huh7 cells with FFA. d–f Effect of omentin-1 treatment on the NF-κB, ERK, and JNK mRNA expression in Huh7 cells with FFA. g, h Effect of omentin-1 treatment on ER stress markers BiP and CHOP in Huh7 cells with FFA. i–l Effects of omentin-1 treatment on the production of ROS in Huh7 with FFA. Values presented are the mean ± SD of at least three biological replicates. GSH and GSSG contents were normalized by the total proteins present in the cell lysates (µg) assessed using BCA. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001