Fig. 7

PepN-elevated CD11c^high macrophages promote Treg differentiation. a, b Flow cytometric analysis indicating proportions of Treg cells in lungs under the indicated conditions (n = 5–6). c mRNA relative levels of Tgf-β, Il-2 and Il-15 in CD11c^high macrophages. d, e Flow cytometric analysis of PD-L1 (d) and CD80 (e) expression in F4/80⁺CD11c^high/int/−macrophages in lungs, respectively (n = 4–5). f Flow cytometric analysis of (%) Foxp3⁺Tregs in CD4⁺ T cells (n = 4). Statistical analysis by one-way ANOVA with Tukey’s multiple-comparison test (b–e), and a two-tailed student’s t test (f). Data are represented as mean ± SD (b–f). ns not significant