Fig. 4

Therapeutic effects of NMN in septic mice. After an hour of FIP (1.8 g feces/kg body weight), mice received a single dose of NMN (500 mg/kg, i.p.) or vehicle. Twenty-four hours after FIP, myocardial function was assessed by echocardiography (A and B). (C) Lung tissues were fixed, embedded and sectioned. H&E staining were performed. A representative microphotograph for H&E staining from 8 lung samples is presented. D MPO activity in lung tissues. E The ratio of wet lung/dry lung. F and G AST and ALT in serum. (H and I) Creatinine and BUN in serum. Data are mean ± SD, n = 8 mice in each group. *P < 0.05 vs saline + vehicle and #P < 0.05 vs feces + vehicle (One-way ANOVA followed by Newman–Keuls test). J A total of 50 mice (male at age 2 months, 25 mice in each group) were monitored for survival for 24 h after FIP (1.8 g feces /kg body weight). *P < 0.05 (the log-rank test). K A total of 44 mice (male at age 2 months) received an intraperitoneal injection of feces (0.8 g/kg). After one hour of FIP, septic mice were allocated into two groups: treatment with vehicle (22 mice) or NMN (100 mg/kg, 22 mice) every other day for 30 days. The survival was monitored. *P < 0.05 (the log-rank test)