Fig. 2

C3a synergized with TGF-β to promote the activation of the NLRP3 inflammasome. A Western blot of NLRP3 and Caspase-1–20 of TCMK-1 cells induced by TGF-β, C3a, and C3a combined with TGF-β. B Quantitative analysis of NLRP3. C Quantitative analysis of Caspase-1–20. D ELISA of IL-1β of TCMK-1 cells induced by TGF-β, C3a, and C3a combined with TGF-β. E IL-1β immunohistochemistry of kidney sections of WT mice (sham operation/UUO). F IL-1β-positive area. Scale bar:25 μm.**P < 0.01;NS, No significance