Fig. 3

CoQ10@TNPs ameliorated oxidative stress and mitochondrial injury in cold I/R-injured H9c2 cells. A, B ROS content in H9c2 cells measured by DHE staining. C, D mtROS content in H9c2 cells measured by MitoSOX staining. E, F Fluorescence intensity of calcein AM in H9c2 cells measured by FCM. G, H MMP was measured by JC-1 staining. Data are represented as the mean ± SD (n = 3). ns: no significance; *p < 0.05; **p < 0.01; ***p < 0.001