Fig. 4

circKIF18B_003 upregulates ACACA by sponging miR-370-3p. A KEGG analyses of circKIF18B_003-targeted mRNAs. The main signaling pathways, including MAPK signaling, PI3K-AKT signaling, and AMPK signaling, are shown in the bubble chart. B Diagram illustrating the overlap between circKIF18B 003-targeted mRNAs and mRNAs in the AMPK signaling pathway. C Schematic depicting the overlapping potential targets of miR-370-3p predicted by StarBase and CircBank databases and upregulated mRNAs differentially expressed between PCa tissues and corresponding normal tissues. D Schematic illustration exhibiting the overlapping screened miR-370-3p targeted genes and mRNAs in the AMPK signaling pathway. E Schematic illustration exhibiting the putative binding site of miR-370-3p in ACACA mRNA predicted by StarBase version 3.0. F The luciferase activity of GP-miRGLO-ACACA in HEK-293T cells after co-transfection with miR-370-3p mimics. G, H Relative ACACA mRNA expression in DU145 and PC-3 PCa cell lines. I Relative ACACA mRNA expression in DU145 cells after knockdown of circKIF18B_003. J Relative ACACA mRNA expression in PC-3 PCa cell line after overexpression of circKIF18B_003. K Relative ACACA protein expression in DU145 and PC-3 PCa cell lines transfected with miR-370-3p mimics. L Relative ACACA protein expression in DU145 and PC-3 cell lines after knocking down and overexpressing the expression of circKIF18B_003