Fig. 6
Dysregulation of the GATA6-AS1/SNAI1 axis is responsible for the malignant behaviors elicited by hypoxia in PDAC. A RT-qPCR and western blotting analysis of SNAI1 expression in Panc-1 cell lines transfected with the indicated vectors or control under 1% O2 conditions. B, C MTT and Edu assays analysis of cell viability in Panc-1 cells transfection with the indicated vectors under 1% or 20% O2 conditions. D, E The wound healing and transwell assay analysis of cell migration and invasion in Panc-1 cell lines transfected with the indicated vectors under 1% or 20% O2 conditions. F, G RT-qPCR and western blotting analysis of E-cad, Vim, ZEB1 and TWIST1 expression in Panc-1 cells with transfection of the indicated vectors under 1% or 20% O2 conditions. H Panc-1cell lines transfected with the indicated vectors were injected into the right dorsal of nude mice. Tumor volume curve and tumor weight of subcutaneous xenografts derived from mouse was analyzed. I The tumor sections were subjected to immunohistochemistry staining using antibodies against ki-67. Scale bar, 100 μm. Data represent mean ± S.D. from three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001