Fig. 4
GATA6-AS1 suppresses EMT under hypoxia through inhibiting SNAI1 mRNA stability. A, B Immunohistochemical staining and RT-qPCR analysis of SNAI1 expression in human PDAC tissues in cohort A and cohort B, respectively. The R-values and P-values were derived via Spearman correlation analysis. Scale bar, 200 μm. C Luciferase reporter assay analysis of SNAI1 promoter activity in Panc-1 and BxPC 3 cells with transfection of GATA6-AS1 overexpression vector or control. D Cellular fractionation experiments analysis of SNAI1 RNA subcellular distribution of PDAC cells transfection with the indicated vectors. E Western blot assays analysis of SNAI1 protein in PDAC cells with MG132 treatment. PDAC cells were transfected with the indicated vectors and exposed to 1% or 20% O2. F, G RNA stability assay analysis of SNAI1 mRNA in PDAC cells with Actinomycin D treatment. PDAC cells were transfected with the indicated vectors and exposed to 1% or 20% O2. Data represent mean ± S.D. from three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001