Fig. 2
Overexpression of GATA6-AS1 inhibits hypoxia-induced PDAC tumorigenesis and EMT in vitro and in vivo. A RT-qPCR and western blot assays analysis of GATA6-AS1 in PDAC cell lines transfected with GATA6-AS1 or pcDNA3.1. B–G The MTT (B), Edu (C), wound healing (D, E) and transwell (F, G) assay analysis of cell viability, migration and invasion in Panc-1 and BxPC 3 cell lines transfected with GATA6-AS1 or pcDNA3.1 under 1% or 20% O2. H, I RT-qPCR and western blot assays analysis of EMT markers in PDAC cell lines transfected with GATA6-AS1 or pcDNA3.1 under 1% or 20% O2. J, K GATA6-AS1-overexpression or control-transfected Panc-1 and BxPC 3 cell lines (5 × 106 per mouse) were injected into the right dorsal of nude mice. Tumor volume curve and tumor weight of subcutaneous xenografts was analyzed. L, M The tumor sections were subjected to immunohistochemistry staining using antibodies against ki-67, E-cad and Vim. Scale bar, 100 μm. Data represent mean ± S.D. from three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001