Fig. 5

Interstitial pneumonia (IP)-mediated changes in the tumor microenvironment A Flow cytometric strategy to define cancer cells, tumor-associated macrophages TAM cancer-associated myofibroblasts, CAMF tumor-infiltrating lymphocytes ,TIL regulatory T cells (Treg), and cytotoxic T lymphocytes (CTL). The injected cancer cells were LLC cells. B Representative gating image of cancer cells (GFP + /CD45-) in all lung cells. C Comparison of cancer cell fraction among the LC, IP + LC, and AsA + IP + LC groups on day 28 (n = 6/group). *P < 0.05, **P < 0.01. AsA, ascorbic acid. D Representative gating image of TAM (GFP-/CD45 + /CD206 +) in all lung cells. E Comparison of TAM fraction among the LC, IP + LC, and AsA + IP + LC groups on day 28 (n = 5–6/group). *P < 0.05. F Representative gating image of CAMF (CD140a + /αSMA +) in lung GFP-/CD45-/CD31-/CD326- cells. G Comparison of CAMF fraction among the LC, IP + LC, and AsA + IP + LC groups on day 28 (n = 5–6/group). NS represents not significant. *P < 0.05. H Representative gating images of CTL (CD8 +) in TIL (GFP-/CD3 +) and Tregs (CD25 +) in CD4 + TIL. I–K Comparison of I Treg fraction, J CTL fraction, and K CTL/Treg ratio among the LC, IP + LC, and AsA + IP + LC groups on day 28 (n = 6/group). NS represents not significant. *P < 0.05. **P < 0.01. L Correlations between HIF-1α expression in the tumor tissue and cancer cell fraction, TAM fraction, CAMF fraction, and CTL/Treg ratio on day 28. HIF-1α, hypoxia-inducible factor 1α