Fig. 4

Ex-vivo evaluation of the complement system activation at tumor site. A Upper panel: IF to evaluate the accumulation of IgM in the two experimental groups (AT101 treated mice and unspecific IgM treated mice). In green the signal related to the IgM and in blue the nuclei. Scale bar: 100 µm. Lower panel: bar chart representing the quantification of fluorescence, data are expressed as normalized fluorescence (protein/nuclei). B Upper panel: IF to evaluate C1q complement protein in the two experimental groups (AT101 treated mice and unspecific IgM treated mice). In green the signal related to C1q protein and in blue the nuclei. Scale bar: 100 µm. Lower panel: bar chart representing the quantification of fluorescence, data are expressed as normalized fluorescence (protein/nuclei). C Upper panel: IF to evaluate C3 complement protein in the two experimental groups (AT101 treated mice and unspecific IgM treated mice). In green the signal related to C3 protein and in blue the nuclei. Scale bar: 100 µm. Lower panel: bar chart representing the quantification of fluorescence, data are expressed as normalized fluorescence (protein/nuclei). D Upper panel: IF to evaluate C9 complement protein in the two experimental groups (AT101 treated mice and unspecific IgM treated mice). In green the signal related to C9 protein and in blue the nuclei. Scale bar: 100 µm. Lower panel: bar chart representing the quantification of fluorescence, data are expressed as normalized fluorescence (protein/nuclei)