Fig. 1

In-vitro characterization of AT101. A Schematic representation of GPC1 protein and visualization of the aminoacids employed for the mouse immunization. B SDS-PAGE analysis performed in non-reducing and reducing condition to determine the IgM nature of AT101. C Elisa to assess the GPC1 binding activity of AT101, the positive control (CTRL +) was represented by serum of mice immunized with GPC1, the negative control (CTRL-) was performed by only using the goat anti-mouse IgG/IgM antibody conjugated with alkaline phosphatase (AP) without AT101 (n = 3). D Flow cytometry analysis to evaluate GPC1 expression in BXPC3 and Jurkat cells using AT101 and the commercial anti-GPC1 antibody as positive control (n = 3). ***, 0.001 < p ≤ 0.0001