Table 1 Primer sequences used for qPCR examination
From: Paneth cell-derived iNOS is required to maintain homeostasis in the intestinal stem cell niche
Gene name | Forward primer | Reverse primer |
|---|---|---|
Actb | CTTCTTTGCAGCTCCTTCGTT | TTCTGACCCATTCCCACCA |
Nos2 | CACAGAGGGCTCAAAGGAGG | AAAGTGGTAGCCACATCCCG |
Lgr5 | CAGGTCAATACCGGAGCGAG | GCGAGGCACCATTCAAAGTC |
MKi67 | ATCATTGACCGCTCCTTTAGGT | GCTCGCCTTGATGGTTCCT |
Lyz2 | ATGGAATGGCTGGCTACTATGG | ACCAGTATCGGCTATTGATCTGA |
Wnt3a | TGGAACTGTACCACCATAGATGAC | ACACCAGCCGAGGCGATG |
Cela2a | TACCCCACTTATGAGGTGGAG | GTCTGATAGTTGCTGAGGCAAT |
Prss2 | TATCAGGTGTCCCTAAATGCTGG | GGATGCGGTATTTGTAGCAGT |
Try5 | GAATTCACTCCTGTTCCTGGC | CAGAGACACCTGGTAGGGGA |
Cel | CGCCTGGAGGTTCTATTTCTTG | GCCCTTGAAGATGTCAACAGA |
S100g | ATGTGTGCTGAGAAGTCTCCT | CGCCATTCTTATCCAGCTCCTT |
Krcap3-1 | CTGCCCACATGAGATCAGCC | GGCAAGAGTTGAGCAGCCA |
Bco1 | ATGGGGAGGTCTTCTACAGGA | GATGGTGTGAGACAAGTAGGAGA |
Dll4 | TTCCAGGCAACCTTCTCCGA | ACTGCCGCTATTCTTGTCCC |
H2-Aa | TCAGTCGCAGACGGTGTTTAT | GGGGGCTGGAATCTCAGGT |
H2-DMb1 | ACCCCACAGGACTTCACATAC | GGATACAGCACCCCAAATTCA |
H2-Ab1 | AGCCCCATCACTGTGGAGT | GATGCCGCTCAACATCTTGC |
Nod2 | CAGGTCTCCGAGAGGGTACTG | GCTACGGATGAGCCAAATGAAG |
Pck1 | CTGCATAACGGTCTGGACTTC | CAGCAACTGCCCGTACTCC |
G6pc1 | CGACTCGCTATCTCCAAGTGA | GTTGAACCAGTCTCCGACCA |
Atf3 | GAGGATTTTGCTAACCTGACACC | TTGACGGTAACTGACTCCAGC |
Fbp1 | CACCGCGATCAAAGCCATCT | AGGTAGCGTAGGACGACTTCA |