Fig. 5

iNOS deficiency inhibited the growth of enteroids. A Representative results for the growth of enteroids derived from wild-type and iNOS^−/− mice on days 1, 3, and 7 after seeding. Bar = 200 μm. B The enteroid formation efficiency of the iNOS^−/− group was clearly decreased compared to that of the control group at 24 h after culture. C The budding numbers of enteroids between the control group and iNOS^−/− group were divided into 0, 1–3 or > 3 per organoid. iNOS^−/− group had less budding capacity. D RNA samples from the enteroids of the two groups at Day 4 were used for qPCR analysis of Lgr5, MKi67, Lyz2 and Wnt3a. n.s: P > 0.05, *P < 0.05, n = 3. E Enteroids derived from the control and iNOS^−/− groups in Lgr5-tdTomato mice on day 1 after seeding. Bar = 50 μm. F Cultured enteroids were treated with 1400W, and the growth was significantly inhibited. Bar = 200 μm. G Areas of enteroids in the two groups. *P < 0.05