Fig. 4
From: Paneth cell-derived iNOS is required to maintain homeostasis in the intestinal stem cell niche
iNOS deletion inhibited the quantity and differentiation of AISCs in the intestinal epithelium. A Immunohistochemistry staining of Olfm4 in ileum tissues from the control group and iNOS−/− group. Bar = 50 μm. B Statistical analysis of Olfm4-positive cells between the two groups in Fig. 4A. C qPCR analysis of the mRNA level of Olfm4 gene in ileum samples. *: P < 0.05. D Confocal images of tdTomato (red), BrdU (green) and DAPI (blue) positive signals in Lgr5-tdTomato and Lgr5-tdTomato; iNOS−/− littermate mice. Bar = 100 μm. E Comparison of tdTomato-positive cells in villus or crypts in the control and iNOS−/− groups. F Respective analysis of BrdU+ cells in the lower 1/3 or the upper 2/3 of crypts. n.s: P > 0.05, **P < 0.01, ****P < 0.0001, n = 3