Fig. 1

Distribution and localization of iNOS in the small intestine of mice. A Representative IHC staining images of iNOS in different intestinal segments from wild-type mice. Bar = 100 μm. B, C Quantification of the staining intensity of iNOS and the number of iNOS positive cells in different segments. *P < 0.05, **P < 0.01, ***P < 0.001. D qPCR analysis of iNOS mRNA levels in different intestinal segments. The ileum had the highest level of iNOS mRNA, and the differences in iNOS expression between the duodenum vs. Jejunum, Duodenum vs. Colon and Jejunum vs. Ileum were not significant (P > 0.05). *P < 0.05, **P < 0.01, n = 3. E qPCR analysis of iNOS mRNA expression in the whole small intestine and crypts. F Lgr5-tdTomato (red), iNOS (green) and DAPI (blue) positive signals at the cryptal base of Lgr5-tdTomato mice. G Lysozyme granules (red), iNOS (green) and β-catenin (purple) positive signals at the crypt base of wild-type mice. H Cultured wild-type enteroid was stained by lysozyme (red), iNOS (green) and DAPI (blue) at day 4. Bar = 20 μm