Fig. 8

E2F-1 directly binds to the MAD2L2 promoter to promote increased expression. A The correlation between MAD2L2 and E2F1 in TCGA, CGGA325, and CGGA693. B MAD2L2 expression levels consistently exhibit a significant positive correlation with low p-values in the 33 cancer tissues analyzed from TCGA. C, D the expression of MAD2L2 at both protein and mRNA levels changed concordantly with alterations in E2F-1 expression. E Dual-luciferase reporter gene assays confirmed E2F-1 enhanced the transcriptional activity of MAD2L2. F Schematic diagram of promoter binding sites. G Agarose gel electrophoresis confirmed significant binding of E2F-1 to binding site 3. H ChIP-qPCR further confirmed this binding in E2F-1 knockdown and overexpression cell lines. I Mutations in the sequence of binding site 3 eliminated the changes in luciferase intensity caused by E2F-1 overexpression or knockdown. *P < 0.05, **P < 0.01, ***P < 0.001