Fig. 7

MAD2L2 promote the malignant behaviors of glioma cells through c-MYC. A GSEA analysis reveals that MAD2L2 is associated with the activation of the MYC pathway. B A heatmap displays the expression correlation between MAD2L2 and MYC in 13 glioma GEO sequencing datasets. C, D knockdown of MAD2L2 resulted in decreased expression of c-MYC, while overexpression led to its upregulation. E, F The decreased proliferative capacity of glioma cells after MAD2L2 knockdown can be reversed by c-MYC overexpression. Similarly, overexpression of MAD2L2 cannot enhance the proliferative capacity of glioma cells after c-MYC knockdown. G, H Overexpression of c-MYC can reverse the damage caused by MAD2L2 knockdown on the migration and invasion abilities of glioma cells. Conversely, overexpression of MAD2L2 followed by c-MYC knockdown does not increase the migration and invasion abilities of the cells. I treatment of c-MYC reversed the alterations in stem cell sphere-forming ability caused by MAD2L2. *P < 0.05, **P < 0.01, ***P < 0.001