Fig. 6

MAD2L2 affects the stemness of glioma cells. A In the MAD2L2 high-expression group, all six different stemness scores are higher compared to the low-expression group. B GSEA analysis demonstrates that MAD2L2 is associated with the activation of glioblastoma stem cell pathways. C The expression of MAD2L2 is higher in stem cells compared to non-stem cells. D Immunofluorescence of CD133 and NESTIN in U87-derived glioblastoma stem cells. E After MAD2L2 knockdown, the sphere-forming ability of glioblastoma stem cells is reduced. Conversely, when MAD2L2 is overexpressed, the sphere-forming ability of glioblastoma stem cells is enhanced. F, G MAD2L2 knockdown downregulated the protein levels of stemness biomarkers, while MAD2L2 overexpression upregulated them. *P < 0.05, **P < 0.01, ***P < 0.001