Fig. 5
From: Inhibition of ferroptosis reverses heart failure with preserved ejection fraction in mice
Ferrostatin-1 inhibits ferroptosis and alters the expression levels of ferroptosis hub DEGs. A Experimental design. B Relative expression of Ptgs2 mRNA in myocardial tissues using RT-qPCR. Gapdh was used as an internal reference, n = 5 mice per group. C Representative Prussian blue-stained images of cross-sections of two groups of mouse hearts, and black arrows show blue-stained cardiomyocytes; Percentage of Prussian blue-stained positive cells, n = 5 mice per group. D Measurement of total iron ion content in myocardial tissue, n = 5 mice per group. E SOD activity level in myocardial tissue, n = 5 mice per group. F Representative DHE staining images of heart tissue, n = 5 mice per group. G Representative transmission electron microscopy images of cardiac tissue, n = 5 mice per group. H, I MDA levels in plasma and heart tissue, n = 5 mice per group. J Representative western blot images and average protein levels of SLC7A11, GPX4, NRF2 and ATF4 in HFpEF+Veh, HFpEF+Fer-1 and HFpEF+DFP mouse hearts, n = 5 mice per group. K Ferroptosis-associated hub DEGs mRNA expression in HFpEF+Veh and HFpEF+Fer-1 mice. Statistical significance was calculated by one-way or two-way ANOVA; *P < 0.05, **P < 0.01, and ***P < 0.001