Fig. 2

GCNT3, MUC1, and MUC5AC expression in IPMN and PDAC patients. a GCNT3 gene expression values (normalized TPM) from tumor and adjacent normal tissues were reported. T-Student test p < 0.001; b Pearson’s R correlation coefficient to assess relationships between the mRNA expression levels of GCNT3, MUC1 and MUC5AC genes. P < 0.05 was considered as statistically significant (GCNT3 Vs MUC1, Cor = 0.48; GCNT3 Vs MUC5AC, Cor = 0.51; MUC1 Vs MUC5AC, Cor 0.57); c survival probability curves for TCGA data. Kaplan–Meier survival probability curves for log survival time in the TCGA PDAC data patients. Patients predicted to have long survival times (in black) and for the patients predicted to have short survival times (in red). GCNT3, HR = 1.64 (1.02 < CI < 2.63), P = 0.039; MUC5AC, HR = 1.64 (1.31 < CI < 3.18), P = 0.0013; MUC1, HR = 2.56 (1.45 < CI < 4.54), P = 0.00081; d Multiplex IF analysis for PanCK, GCNT3, MUC1, MUC5AC, CD4, CD8, CD163, CD68. Images shown are representative of 1 out of more than 20 fields acquired. e Plot showing the counts of positive cell per area (20 ROIs). Kruskal–Wallis test was used to compare differences between sample groups with Low-grade IPMN as control. *** = Pvalue < 0.01, ** = 0.01 > Pvalue < 0.05, * = 0.025 > Pvalue < 0.05. (LG-IPMN) lowgrade IPMN, (HG-IPMN) High-grade IPMN, (PDAC) Pancreatic Ductal Adenocarcinoma