Fig. 1

GeoMX spatial transcriptomics analysis. a Low-grade (LG), and High-grade (HG) IPMN FPPE samples were stained with PanCk (green) markers for tumor cells, and CD45 (orange) for immune cells detection. Syto13 was used as nuclear staining (blue). b GeoMX data was analyzed with Seurat and differential expression analysis was performed for HG compared to LG IPMN. c, d Mucin genes were consistently overexpressed together with the genes responsible for their O-glycosylation. e We calculated module score for the mucin O-glycosylation signature obtained from Reactome using the addmodulescore() function from Seurat. HG IPMNs showed consistent higher scores in respect to LG, and borderline IPMNs