Fig. 6
From: TC2N inhibits distant metastasis and stemness of breast cancer via blocking fatty acid synthesis
TC2N decreases the levels of PTEN neddylation by preventing the nuclear import of PTEN. A M231 cell extracts were subjected to IP with anti-PTEN antibody and further analyzed by WB with anti-NEDD8 antibody. Normal IgG was used as a negative control. Whole-cell lysates were used as a positive control (Input). B Immunofluorescence revealed that TC2N decreases the nuclear localization of PTEN in MCF7 cells. Scale bars represent 25 μm. C PTEN protein level in nucleus and cytoplasm were analyzed by WB. D The extracts from BC cells were subjected to IP with anti-Flag antibody and further analyzed by WB with anti-importinβ and anti-IPO5 antibodies. Normal IgG was used as a negative control. Whole-cell lysates were used as a positive control (Input). E The cell extracts from BC cells were subjected to IP with importinβ antibody and further analyzed by WB with indicated antibodies. Normal IgG was used as a negative control. Whole-cell lysates were used as a positive control (Input)