Fig. 4

TC2N decreases the fatty acid content in BC. A CPTAC BC protein dataset identified the association between TC2N expression and lipid metabolism. B Effects of TC2N overexpression on total free fatty acids levels of M231, MCF7 and SKBR3 cells. The P value was measured with Student’s t-tests. **P < 0.01, ***P < 0.001. C Lipid metabolism was detected by LC–MS/MS in stable transfected M231 cells. Heatmap was performed and the significant differences were analyzed using Student’s t-test, n = 4. D The growth of E0771 cells stably expressing control lentivirus or TC2N overexpression lentivirus in nude mice. Block arrows indicate the tumor masses. E The fatty deposit of E0771 tumor masses were detected by oil red O staining. Scale bar represent 100 μm. F Immunofluorescence analysis of the localization of TC2N and FASN in MCF7-TC2N cells. DAPI serves as a nuclear counterstain. Scale bars represent 25 μm. G Immunofluorescence revealed that TC2N decreases the protein levels of FASN in MCF7 cells. Scale bars represent 25 μm. H qRT-PCR revealed that TC2N do not change the mRNA expression of FASN in M231 and MCF7 cells. The P value was measured with Student’s t-tests. ns, no significance. I WB revealed that TC2N decreases the protein expression of FASN in M231 and MCF7 cells