Fig. 3

TC2N interferes with the CSC-like characteristics of BC cells. A WB analysis of NANOG and SOX2 in stable M231, MCF7 and SKBR3 cells. B Sphere formation ability of BC cells with ectopic expression of TC2N. Mean ± SEM (n = 3). The P value was measured with Student’s t-tests. **P < 0.01. C Fractions of CD44⁺CD24⁻ BC cell-spheres with TC2N ectopic expression were determined by flow cytometry. Mean ± SEM (n = 3). The P value was measured with Student’s t-tests. ***P < 0.001. D Fractions of ADLH⁺ BC cells with TC2N ectopic expression were determined by flow cytometry. Mean ± SEM (n = 3). The P value was measured with Student’s t-tests. **P < 0.01, ***P < 0.001. E The tumor growth curves of M231 spheres (n = 5). *P < 0.05. F The image of nude mice subcutaneously injected with M231 spheres. G Tumor weight from the Vc and TC2N group. **P < 0.01. H IHC evaluation of CD133 in xenograft tumor. Scale bar represent 100 μm