Fig. 2

TC2N inhibits metastatic phenotype of BC cells in vitro and in vivo. A Overexpression of TC2N in M231, MCF7 and SKBR3 cells were examined by WB. B Effects of TC2N overexpression on migration and invasion of M231, MCF7 and SKBR3 cells were detected by transwell assays. Mean ± SEM (n = 3). The P value was measured with Student’s t-tests. **P < 0.01, ***P < 0.001. C Silencing of TC2N in M231-TC2N, MCF7-TC2N and SKBR3-TC2N stable transfectants were examined by WB. D Effects of TC2N knockdown on migration and invasion of M231-TC2N, MCF7-TC2N and SKBR3-TC2N cells were detected by transwell assays. Mean ± SEM (n = 3). The P value was measured with Student’s t-tests. *P < 0.05, **P < 0.01, ***P < 0.001. E Bioluminescent images of brain and heart tissues from female nude mice 50 days post intracardiac injection of SKBR3 cell line with TC2N ectopic expression. F Tissues were exfoliated from mice (n = 4) that injected with stable transfected SKBR3 cells. Organs invaded by BC cells were evaluated by H&E staining (Left). Scale bar represent 100 μm. The number of metastatic nodules in each organ were showed (Right). The P value was measured with Student’s t-tests. *P < 0.05