Fig. 7

miR-4687-5p was a novel suppressor of FGFR1 by targeting FGFR1 3′UTR and CDS. A Bioinformatics analysis by TargetScan, miRwalk, Microt4, miRanda and RNAhybrid databases revealed two potential miR-4687-5p-interacting sites located in FGFR1 3′UTR and CDS, respectively. B The miR-4687-5p binding sequences in 3′UTR (5′-AGGGCU-3′, red) and CDS (5′-GAGGGCTG-3′, blue) were highly conserved across species. C Artificial overexpression of miR-4687-5p by mimics significantly suppressed expression of FGFR1 mRNA and protein. D Dot blot hybridization showing biotin-labelled miR-4687-5p probe bound to wild-type FGFR1 3′UTR and CDS fragments in a dose-dependent manner, while mutation of miR-4687-5p binding sites in FGFR1 mRNA (FGFR1 3′UTR-MUT and FGFR1 CDS-MUT) resulted in no or signals. Error bars for qRT-PCR represented mean ± standard deviation (SD) of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001