Fig. 6
miR-4687-5p was downregulated in PCa and interacted with circFGFR1int2. A Bioinformatics analysis (RegRNA 2.0 and RNA22 v2) of hsa-miR-4687-5p showing the potential circFGFR1int2-interacting sequence TGTGGGGTGAGGGCT (in red) at 762 to 779. B, C miR-4687-5p was significantly down-regulated in PCa cells (LNCap, PC-3, and DU145) and tissue samples (n = 6) than in normal prostate cell RWPE-1 and BPH tissues. D Expression of FGFR1 mRNA was negatively correlated with that of miR-4687-5p. E Dot blot hybridization showed biotin-labelled miR-4687-5p probe bind to wild-type circFGFR1int2 (WT) in dose-dependent manner, whereas mutation of the binding site (MUT) resulted in no or very weak signals. Blank (no RNA was added) was used as negative control. Error bars for qRT-PCR represented mean ± standard deviation (SD) of three independent experiments. **P < 0.01, ***P < 0.001, ns, not significant