Fig. 3
Analysis and identification of circFGFR1int2-interacting proteins. A Analysis by catRAPID, circAtlas, RBPDB and RBPmap databases revealed potential circFGFR1int2-binding proteins and enriched GO terms and KEGG pathways. B Analysis by Metascape database (http://metascape.org/gp/index.html#/main/step1) showed potential protein–protein interactions of the circFGFR1int2-interacting proteins and the biological processes they may be involved in. C The FUS protein was identified as a circFGFR1int2-interacting protein by all four databases. D RNA immunoprecipitation (RIP) showed retrieval of circFGFR1int2 from complexes obtained with both anti-P65 and anti-FUS. Knockdown of circFGFR1int2 by ASO-circFGFR1int2 decreased recovery of circFGFR1int2. Non-immune-IgG was used as negative control. E FUS and P65 were pulled down by biotin-labeled circFGFR1int2 probe, knockdown of which decreased the recovery. F Co-immunoprecipitation (Co-IP) showed retrieval of P65 by anti-FUS, and FUS by anti-P65, respectively. Non-immune-IgG was used as negative control. G Expression of P65 and FGFR1 in PCa tissue samples was significantly correlated (TCGA, n = 492, R = 0.29, P < 0.001)