Fig. 2
CircFGFR1int2 promoted expression of FGFR1 mRNA and protein. A CircFGFR1int2 and FGFR1 mRNA levels were significantly higher in PC-3, DU145, LNCaP, and 22Rv1 PCa cells than in normal prostate RWPE-1 cells. B CircFGFR1int2 and FGFR1 mRNA were significantly up-regulated in PCa (n = 6) than in BPH (n = 5) tissue samples. C Expression of circFGFR1int2 and FGFR1 mRNA in PCa cells and tissues was significantly correlated. D–F Knockdown of circFGFR1int2 by ASO-circFGFR1int2 significantly reduced expression of FGFR1 mRNA (D, RT-PCR; E, qRT-PCR) and protein (F, Western blot), whereas artificial overexpression by OE-circFGFR1int2 rescued FGFR1 expression. Error bars for RT-PCR, qRT-PCR, and Western blot represented mean ± standard deviation (SD) of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001