Fig. 6

CircTENM3 suppresses PCa progression in vivo. A Photographs of tumors obtained from the Ad-NC and Ad-circTENM3 groups of nude mice. N = 6. B Tumor volumes were measured once a week after tumor formation. C Tumors were removed and weighed 28 days after implantation. D Immunofluorescence staining of Ki-67 in the tumors obtained from the Ad-NC and Ad-circTENM3 groups of nude mice. N = 6. E qPCR was performed to assess the relative circTENM3, miR-558 and RUNX3. F RUNX3 protein levels of the Ad-NC and Ad-circTENM3 groups. N = 3. G Schematic illustration presented the molecular mechanism of circTENM3 in PCa cells. The downregulation of circTENM3 leaded to an increase of miR558 and the decrease of RUNX3, which promoted the proliferation of PCa cells. All data were presented as the mean ± SD. B Two-way ANOVA with Bonferroni post hoc test. C, D, E, F unpaired Student’s t-test. *P < 0.05