Fig. 5

CircTENM3 enhances RUNX3 levels via miR-558 sponge. A The correlation between circTENM3 and miR-558 in PCa. N = 20. B, C Western blot assay was performed to assess the RUNX3 protein levels in DU145 and PC3 cells with circTENM3 overexpression. N = 3. D The direct binding sites between miR-558 and RUNX3. E Luciferase reporter assay was performed to confirm the direct binding relationship between miR-558 and RUNX3. N = 4. F, G Western blot assay was performed to assess the effect of miR-558 on RUNX3 protein levels in DU145 and PC3 cells with or without circTENM3 overexpression. N = 3. All data were presented as the mean ± SD. C, E, unpaired Student’s t-test. G One-way ANOVA with Bonferroni post hoc test. *P < 0.05