Fig. 3

CircTENM3 acts as a miR-558 sponge. A CircInteractome database showed the predicted downstream targets of circTENM3. B qPCR analysis was used to determine the effect of circTENM3 kncokdown on the predicted downstream targets in DU145 cells. N = 4. C qPCR analysis was used to determine the effect of circTENM3 overexpression on the predicted downstream targets in DU145 cells. N = 4. D–G The association between circTENM3, miR-558 and AGO2 was ascertained by analyzing DU145 cell lysates using RNA immunoprecipitation with an AGO2 antibody. qPCR was used to detect the change of circTENM3 (D, E) and miR-558 (F, G) levels. N = 4. H, DU145 cell lysis was pulled down and enriched with circTENM3 specific probe and then detected the expression of miR-558 by qPCR. N = 4. I, The binding site of circTENM3 and miR-558. J, The direct binding sites between circTENM3 and miR-558. K, Luciferase reporter assay was performed to confirm the direct binding relationship between circTENM3 and miR-558. N = 4. All data were presented as the mean ± SD. B, C, H, K, unpaired Student’s t-test. E, G, One-way ANOVA with Bonferroni post hoc test. *P < 0.05