Fig. 1

Identification and characterization of circRNAs in PCa. A The Volcano plot illustrated the differentially expressed circRNAs in PCa extracted from the GSE140927 dataset. B The cluster heatmap displayed the expression patterns of the 10 most significant upregulated and downregulated differentially expressed circRNAs. C The qPCR analysis examined the 10 downregulated circRNAs levels in PCa, compared to paracancerous tissue. N = 4. D circTENM3 homology analysis in human and mouse genome was conducted using Basic Local Alignment Search Tool. E Schematic presentation exhibiting the origination of circTENM3. F Alterations in the circTENM3 and TENM3 mRNA levels were detected by qPCR after Actinomycin D treatment in DU145 and PC3 cell lines. N = 4. G qPCR analysis of circTENM3 and TENM3 mRNA levels after RNase R treatment in DU145 and PC3 cell lines. N = 4. H The expression of nuclear control transcript (U6), cytoplasm control transcript (GAPDH) and circTENM3 was detected by qPCR in the nuclear and cytoplasm fractions of DU145 and PC3 cell lines. N = 4. I RNA-FISH assays were conducted to detect circTENM3 expression in DU145 and PC3 cell lines using anti-circTENM3 probes. Nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI). Scale bar = 20 μm. All data were presented as the mean ± SD. C, G, unpaired Student’s t-test. F, Two-way ANOVA with Bonferroni post hoc test. *P < 0.05