Fig. 6

Inhibition of EGFR promotes migration of neuroblast and enrichment of neuroblasts within the injured area. Mice were mechanically injured in the primary motor cortex and were injected in the lateral ventricle (LV) with a control lentivirus that express the green fluorescent protein ZsGreen. Mice were treated for 14 days with intranasal administrations of the EGFR inhibitor Afatinib (1 μM) or vehicle. A Representative confocal images of the injured cortex of adult mice treated with vehicle and processed for the detection of DCX and ZsGreen. B Representative confocal images of the injured cortex of adult mice treated with Afatinib showing ZsGreen fluorescence and the immunodetection of DCX. The dotted line indicates the limit of the lesion (L) and the scale bar represents 50 μm. C Graph represents the number of DCX⁺ cells in the perilesional area comparing Afatinib treated mice with vehicle treated mice. D Graph represents the number of DCX⁺ cells that are also, ZsGreen⁺ cells. Statistical analysis: * p < 0.05. Data are the mean ± S.E.M; n = 4–6 animals per group, using a Student´s t-test for equal-variance unpaired samples