Fig. 4

The number of immature SVZ neuroblasts increases in response to a cortical injury. A Representative confocal images of adult mice SVZ bearing a unilateral cortical injury. Mice were sacrificed at 7 days post injury (dpi) and processed for the immunodetection of the early neuroblast marker doublecortin (DCX), the epidermal growth factor receptor marker (EGFR) (upper panel), and of the proliferation marker Ki67 (lower panel). The dotted line indicates the limits of the lateral ventricles (LV). The scale bar represents 50 μm and 25 μm in the high magnification images. B Graph shows the burden of DCX⁺ cells as a percentage of the total SVZ area comparing the ipsilateral to the contralateral site. C Graph shows the number of EGFR⁺ cells that co-express the neuroblast marker DCX/mm³ in the ipsilateral and the contralateral SVZ. D Graph shows the number of Ki67⁺ cells that co-express the neuroblast marker DCX/mm³ in the ipsilateral and the contralateral SVZ. Statistical analysis: * p < 0.05 compared the ipsilateral with contralateral side using a Student´s t-test for equal-variance unpaired samples. Data are the mean ± S.E.M; n = 4–6 animals per group