Fig. 3

The number of proliferative cells increases in the SVZ after a cortical injury. Mechanical cortical lesions were unilaterally performed in the primary cortex of adult male mice. Mice were sacrificed at 7 days post-injury (dpi) and processed for the immunodetection of the epidermal growth factor receptor marker (EGFR), and the proliferation marker Ki67. A Representative confocal images of adult mouse SVZ bearing a unilateral cortical injury showing the immunodetection of EGFR. The dotted line indicates the limits of the lateral ventricles (LV). The scale bar represents 50 μm. B Graph shows the number of EGFR⁺ cells per mm³ in the ipsilateral and the contralateral SVZ. C Representative confocal images of adult mice SVZ bearing a unilateral cortical injury showing the immunodetection of the proliferation marker Ki67. D Graph shows the number of Ki67⁺ cells per mm³ in the ipsilateral and the contralateral SVZ. Statistical analysis: * p < 0.05 compared the ipsilateral with contralateral side using a Student´s t-test for equal-variance unpaired samples. Data are the mean ± S.E.M; n = 4–6 animals per group