Fig. 2

The number of TGF-α⁺ microglial cells increases after a cortical injury in the SVZ and perilesional area. Mechanical cortical lesions were unilaterally performed in the primary cortex of adult male mice. Mice were sacrificed 7 days post-injury (dpi) and processed for the immunodetection of TGF-α and the microglial marker Iba1. A Representative confocal images showing the area surrounding the cortical lesion (ipsilateral) and the non-injured hemisphere (contralateral) processed for the immunodetection of TGF-α and Iba1. The dotted line indicates the limit of the lesion (L) and the scale bar represents 50 μm. B Graph represents the number of TGF-α⁺-Iba1⁺ cells in the perilesional area compared to contralateral cortex. C Representative confocal images of adult mice SVZ bearing a unilateral cortical injury. Ipsilateral and contralateral SVZ were processed for the immunodetection of TGF-α and Iba1. The dotted line indicates the limits of the lateral ventricles (LV). The scale bar represents 50 μm. D Graph shows the number of TGF-α⁺-iba1⁺ cells in the ipsilateral and contralateral SVZ. Statistical analysis: * p < 0.05 compared the ipsilateral side with the contralateral in a Student´s t-test for equal-variance unpaired samples. Data are the mean ± S.E.M; n = 4–6 animals per group