Fig. 7

PTPRH enhanced glycolysis via the phosphatidylinositol-3-kinase (PI3K)/Protein kinase B (AKT)/mammalian target of rapamycin (mTOR) signaling pathway. A GSEA to identify enriched signaling pathways for PTPRH. B Representative immunostaining images after staining of PI3K/AKT/mTOR signaling pathway-related proteins in A549 xenografts. C, D Relative expression levels of PI3K/AKT/mTOR signaling pathway-related proteins in the sh-PTPRH and sh-NC groups by western blotting. E Western blotting results showing the effects of sh-PTPRH and the PI3K activator 740Y-P alone and in combination on the expression levels of glycolysis-related proteins. F Western blotting results showing the effects of OE-PTPRH and the PI3K inhibitor LY294002 alone and in combination on the expression levels of glycolysis-related proteins