Fig. 5

TRPA1/TRPV1 as effector channels for non-histaminergic itch signaling. The involvement of  the ion channels TRPA1 and TRPV1 in itch and pain sensation induced by Beta-Alanine (panels A), BAM 8-22 (panels B) and  Cowhage (panels C) were assesed using respective specific pharmacological blockers A-967079 and BCTC. The first two columns show itch ratings (NRS, values are means ± SEM) for 300 s after the injection of the pruritogens with (black triangles) and without (gray circles) pre-injection of the TRPA1 blocker (A-967079) or the TRPV1 blocker (BCTC). The third shows single values of the AUC for itch ratings provided by each volunteer for control in the middle and with pre-injection of TRPA1 blocker to the left and TRPV1 blocker to the right. Significant differences are indicated (*P < 0.05)