Fig. 3

DMF prevents neuronal cell loss in the hippocampus following SE. A Representative images of the hippocampi of rats subjected to KA-SE and treated with vehicle (n = 6, left) or DMF (200 mg/kg/day over 7 days, n = 6, right). CA1 and CA3 (highlighted in yellow) regions were analyzed for neuronal density 1 week following KA-SE onset. Scale bar = 500 µm. B, D Representative images (zoom view) of the CA1 region (B) and CA3 (D) of animals, as in (A), illustrating the staining of neurons (NeuN, green). The nuclei were stained with DAPI (blue). Scale bars = 50 μm. C, E Quantification of the number of NeuN cells per 1 mm² of tissue in CA1 (C) and CA3 (E). Data are expressed as the mean ± SEM. **P < 0.01 and ***P < 0.001 after one-way ANOVA followed by Tukey’s post hoc test