Fig. 5

Increased m6A methylation level alleviates denervated muscle atrophy. A Schematic diagram of drug injection. B The levels of m6A in skeletal muscle after drug injection, p-value calculated through unpaired t-test, n = 4, **p < 0.01. C Immunofluorescence staining results of skeletal muscles after drug injection, Bar = 50 μm, blue: DAPI, green: laminin. D Statistical results of cross-sectional area of skeletal muscle fibers. E The distribution of muscle fiber cross sectional area after different drug treatments, p-value calculated through unpaired t-test, n = 4, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 vs Inn + DMSO group; ^#p < 0.05, ^##p < 0.01, ^###p < 0.001 vs Den + DMSO group. F Statistical results of the muscle mass/body mass ratio after drug treatment. G Statistical results of skeletal muscle wet weight ratio after drug treatment. H Western blot results of MHC expression after drug treatment. I Statistical results of MHC protein level. J Western blot and statistical results of Alkbh5 and Fto levels after drug treatment. p-value calculated through unpaired t-test, n = 3, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001