Fig. 4

Changes in m6A regulators expression during denervation-induced muscle atrophy. A Immunofluorescence staining of skeletal muscles after denervation for 0 h (Ctrl) and 72 h (Den 3d), Bar = 50 μm, blue: DAPI, green: laminin. B Statistical results of wet weight ratio after denervation for 0 h (Ctrl) and 72 h (Den 3d). C Statistical results of the muscle mass/body mass ratio after 0 h and 72 h of denervation, n = 5. D Statistical results of cross-sectional area of skeletal muscle fibers after 0 h and 72 h of denervation, n = 5. E The qPCR results of several common m6A regulators, Alkbh5, Fto, Mettl3, Mettl14, Virma, and Wtap, during the denervation-induced muscle atrophy, n = 3. F The statistical results of m6A levels in skeletal muscle after 0 h and 72 h of denervation, n = 4. The p values were calculated through unpaired t-test, *p < 0.05, **p < 0.01, ****p < 0.0001. Unmarked columns have no statistical significance