Fig. 3

m6A methylation regulates muscle gene expression after denervation. A The expression levels of constitutive and differential m6A host genes. The violin diagram showed the maximum and minimum values, as well as the histogram of gene expression levels. The box plot in the middle represented the interquartile range. B The statistics of the numbers of differentially expressed genes (blue), differential m6A host genes (yellow), and overlapping genes (green) at different time points. C The proportion of overlapped genes in B among all differential m6A host genes (represented by red dots). D Correlation analysis between the differential m6A gene and its corresponding gene expression at 9 time points in B. The y-axis represented the density of the genes, while the x-axis centered around 0 represented negative correlation on the left and positive correlation on the right. E. For the four time points with the higher proportion in C, the overlapping genes, Erfe and Hspa1l, were differentially expressed genes regulated by m6A at each time point. F Functional analysis of differential m6A host genes at 72 h of denervation. The biological processes of TOP10 were displayed, and the lines indicated the interactions between genes. G Functional analysis of m6A methylated differentially expressed genes at 72 h of denervation. The biological processes of TOP10 were displayed, and the genes were analyzed by PPI. The lines indicated the interactions between genes