Fig. 3

Caprin-1 promotes pancreatic cancer growth. A, B EdU assay verified the role of Caprin-1 on pancreatic cancer cells proliferation. C, D Colony formation showed the effects of sh-Caprin-1 and pcDNA-Caprin-1 on Bxpc-3 and Panc-1 cells growth. E, F The orthotopic tumor growth from day 7 to day 28 after tumor implantation was traced using IVUS. Mice were sacrificed at day 28 after tumor injection and tumor size was measured. G The schedule of building PDx model using two PDAC tissues from patients underwent pancreaticoduodenectomy. H Representative images of high and low expressions of Caprin-1 in PDAC samples by IHC staining. I–K The tumor size was compared between Caprin-1^High and Carpin-1^Low groups in subcutaneous PDx model. The tumor growth curve of subcutaneous PDx was screened from day 0 to day 63 after tumor injection. L, M The orthotopic PDx model was built and tumor volumes were compared between Caprin-1^High and Carpin-1^Low groups