Fig. 6

Interactions between stemness subtypes and ICB treatment in pan-tumors. a Unsupervised hierarchical clustering based on the stemness activity scores of the 18 stemness signatures clustered the baseline pan-tumor patients treated with ICB into three subtypes. b Hypergeometric test collaborates an association between stemness subtypes of ICB pan-tumors and responsiveness of ICB therapy. c Stacked histogram showing differences in responsiveness of the three pan-tumor stemness subtypes to ICB. d Univariate logistic regression shows the effect of the three stemness subtypes on ICB efficacy. e, f Sankey diagram showing sample flow for pre-treatment and on/post-treatment of ICB. Separate presentation for responders (e) and non-responders (f). g t-SNE plot of pre-treatment and post-treatment tumor cells of head and neck squamous cell carcinoma (HNSCC, medium), along with their corresponding cytoTRACE scores (left), and the comparison of these scores between two groups (right)