Fig. 7

Cirsiliol inhibits xenogeneic OS tumor growth in situ. A H&E staining of major organs. Scale: 50 µm. B, C A small animal in vivo optical imaging system was utilized to detect fluorescence intensity values after 14 days of continuous treatment with cirsiliol. D Body weight curves were measured for mice in different treatment groups (n = 3 per group). E Statistical analysis of the final tumor volume and size in different treatment groups. F IHCP analysis of the expression of KI67, PCNA and cleaved CASP3 in tumor tissues and the results from H&E staining in treatment and control groups. Scale bar: 100 µm. Data are expressed as mean ± SD. *p < 0.05; **p < 0.01; ***p < 0.001