Fig. 4

Cirsiliol promotes mitochondrial fission and apoptosis. A U2OS and U2OS/MTX cells were treated with DMSO (20 µM), and then stained with MTRC, respectively. B, C U2OS/MTX cells were treated with various concentrations of cirsiliol, stained with anti-Cyto-C and anti-TOMM20 antibodies, and imaged using co-focused microscope imaging. Scale bars:100 µm. Cyto-C and TOMM20 were quantified using FIJI/ImageJ. Data are expressed as mean ± SD. *p < 0.05; **p < 0.01; ***p < 0.001